Journal: International Journal of Molecular Sciences

Article Title: Improved Wound Healing and Skin Regeneration Ability of 3,2′-Dihydroxyflavone-Treated Mesenchymal Stem Cell-Derived Extracellular Vesicles

doi: 10.3390/ijms24086964

Figure Lengend Snippet: List of antibodies for Western blot.

Article Snippet: Akt1 polyclonal antibody , rabbit , Cusabio (CSB-PA000855).

Techniques: Western Blot, Recombinant

Journal: Journal of Advanced Research

Article Title: Efficient improvement of the proliferation, differentiation, and anti-arthritic capacity of mesenchymal stem cells by simply culturing on the immobilized FGF2 derived peptide, 44-ERGVVSIKGV-53

doi: 10.1016/j.jare.2023.09.041

Figure Lengend Snippet: Antibody list.

Article Snippet: Membrane blocking was performed using 5 % skimmed milk in Tris-buffered saline for 1 h, followed by incubation with the appropriate primary antibodies against total ERK (Cat No. B7074, Assay Biotechnology, Fremont, CA, USA), phosphorylated ERK (Cat No. 9101 s, Cell Signaling Technology, Danvers, MA, USA), total AKT (Cat No. CSB-PA000855, CUSABIO, Houston, TX, USA), phosphorylated AKT (Cat No. CSB-PA008120, CUSABIO), IKKα (Cat No. 11930, Cell Signaling Technology), FGFR1 (Cat No. NBP2-33784, Novus Biologicals, Centennial, CO, USA), phosphorylated FGFR1 (Tyr653, Tyr654) (Cat No. 44-1140G, Invitrogen) polyclonal Antibody, FRS2 antibody (Cat No. 3836–100, BioVision, Waltham, MA, USA), phosphorylated FRS2-alpha (Cat No. 3864S, Cell Signaling) or ACTIN (Cat No. sc-8432, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) overnight at 4 °C.

Techniques: Staining, Plasmid Preparation

Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA-185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator-activated receptor β

doi: 10.3892/etm.2021.9797

Figure Lengend Snippet: Effect of PI3K inhibitor LY294002 on the proliferation and migration of HaCaT keratinocytes. HaCaT keratinocytes were treated with 50 µmol/l PI3K inhibitor LY294002 for 48 h. (A) The phosphorylation level of p-AKT at S473 and T308 and the p-AKT/AKT ratio was determined via western blot analysis. (B) Proliferation of HaCaT keratinocytes was assessed using Cell Counting Kit-8 assay. (C) Colony formation assay was employed to assess the number of colonies in HaCaT keratinocytes. (D) The number of migrated HaCaT keratinocytes was examined using Transwell assay. (E) The protein level of cyclin D1, CDK6, CDK4, MMP-2 and MMP-9 in HaCaT keratinocytes was determined via western blot analysis. * P<0.05 vs. control. p, phosphorylated.

Article Snippet: After blocking with 5% non-fat milk at room temperature for 2 h, the membranes were incubated with the following primary antibodies: Cyclin D1 (1:200; cat. no. ab16663), CDK6 (1:3,000; cat. no. ab151247), CDK4 (1:1,000; cat. no. ab95255), p-AKT-T308 (1:1,000; cat. no. ab8933), AKT (1:500; cat. no. ab8805) (all from Abcam), MMP-2 (1:2,000; cat. no. sc-10736), MMP-9 (1:2,000; cat. no. sc-10737), PPARβ (1:5,000; cat. no. sc-74440), integrin-linked kinase (ILK; 1:2,000; cat. no. sc-20019) (all from Santa Cruz Biotechnology, Inc.), phosphoinositide-dependent protein kinase 1 (PDK1, 1:2,000; cat. no. BA4499), p-AKT-S473 (1:1,000; cat. no. P00024-6), (all from Boster Biological Technology Co., Ltd.) or GAPDH (1:2,500; cat. no. ab9485; Abcam) at 4 ̊C overnight.

Techniques: Migration, Phospho-proteomics, Western Blot, Cell Counting, Colony Assay, Transwell Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA-185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator-activated receptor β

doi: 10.3892/etm.2021.9797

Figure Lengend Snippet: Effect of miR-185 and PPARβ overexpression on the proliferation and migration of HaCaT keratinocytes. HaCaT keratinocytes were transfected with miR-185 and PPARβ overexpression plasmid or their NCs. (A) The protein level of PPARβ, ILK, PDK1, p-AKT-S473, p-AKT-T308 and AKT in HaCaT keratinocytes was determined via western blot analysis. (B) Proliferation of HaCaT keratinocytes was assessed using Cell Counting Kit-8 assay. (C) The number of colonies in HaCaT keratinocytes was determined using colony formation assay. (D) Transwell assay was used to assess the number of migrated HaCaT keratinocytes. (E) The protein level of cyclin D1, CDK6, CDK4, MMP-2 and MMP-9 in HaCaT keratinocytes was assessed via western blot analysis. * P<0.05. PPARβ, peroxisome proliferator-activated receptor β; ILK, integrin-linked kinase; PDK1, phosphoinositide-dependent protein kinase 1; miR, microRNA; NC, negative control; p, phosphorylated.

Article Snippet: After blocking with 5% non-fat milk at room temperature for 2 h, the membranes were incubated with the following primary antibodies: Cyclin D1 (1:200; cat. no. ab16663), CDK6 (1:3,000; cat. no. ab151247), CDK4 (1:1,000; cat. no. ab95255), p-AKT-T308 (1:1,000; cat. no. ab8933), AKT (1:500; cat. no. ab8805) (all from Abcam), MMP-2 (1:2,000; cat. no. sc-10736), MMP-9 (1:2,000; cat. no. sc-10737), PPARβ (1:5,000; cat. no. sc-74440), integrin-linked kinase (ILK; 1:2,000; cat. no. sc-20019) (all from Santa Cruz Biotechnology, Inc.), phosphoinositide-dependent protein kinase 1 (PDK1, 1:2,000; cat. no. BA4499), p-AKT-S473 (1:1,000; cat. no. P00024-6), (all from Boster Biological Technology Co., Ltd.) or GAPDH (1:2,500; cat. no. ab9485; Abcam) at 4 ̊C overnight.

Techniques: Over Expression, Migration, Transfection, Plasmid Preparation, Western Blot, Cell Counting, Colony Assay, Transwell Assay, Negative Control

Journal: Scientific Reports

Article Title: Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle

doi: 10.1038/s41598-021-94709-0

Figure Lengend Snippet: Real-time PCR gene expression of Akt, p70S6K, 4E-BP1, and muscle-specific E3 ubiquitin ligases MAFbx and MuRF1. US unstretched, St stretched. Values are expressed as mean ± SEM. *P < 0.05, n = 8/group.

Article Snippet: After blocking with a blocking buffer (Boster Biological Technology, Pleasanton, CA, USA) for 1.5 h at room temperature, the membrane was incubated with primary antibodies (diluted in TBS-T) against p-p70S6K (Thr 389) (1:1,000; A0533; Assay Biotechnology Company, Sunnyvale, CA, USA) and p70S6K (1:1,000; CSB-PA003686; CUSABIO, Houston, TX, USA), p-4E-BP1 (Thr37/46) (1:1,000; #2855; Cell Signaling technology, Danvers, MA, USA) and 4E-BP1 (1:1000; CSB-PA007994; CUSABIO), p-AKT (S473) (1:1000; CSB-PA000466; CUSABIO), AKT (1:1000; CSB-PA008132; CUSABIO), GAPDH (1:1000; CSB-PA004666; CUSABIO) for 2 h at room temperature.

Techniques: Real-time Polymerase Chain Reaction, Gene Expression, Ubiquitin Proteomics

Journal: Scientific Reports

Article Title: Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle

doi: 10.1038/s41598-021-94709-0

Figure Lengend Snippet: The phosphorylation levels of Akt, p70S6K, and 4E-BP1 measured with Western blotting. Representative western blot images and quantification of phosphorylation levels of ( A ) Akt, ( B ) p70S6K, and ( C ) 4E-BP1. US unstretched, St stretched. Values are expressed as mean ± SEM. *P < 0.05, n = 7/group.

Article Snippet: After blocking with a blocking buffer (Boster Biological Technology, Pleasanton, CA, USA) for 1.5 h at room temperature, the membrane was incubated with primary antibodies (diluted in TBS-T) against p-p70S6K (Thr 389) (1:1,000; A0533; Assay Biotechnology Company, Sunnyvale, CA, USA) and p70S6K (1:1,000; CSB-PA003686; CUSABIO, Houston, TX, USA), p-4E-BP1 (Thr37/46) (1:1,000; #2855; Cell Signaling technology, Danvers, MA, USA) and 4E-BP1 (1:1000; CSB-PA007994; CUSABIO), p-AKT (S473) (1:1000; CSB-PA000466; CUSABIO), AKT (1:1000; CSB-PA008132; CUSABIO), GAPDH (1:1000; CSB-PA004666; CUSABIO) for 2 h at room temperature.

Techniques: Phospho-proteomics, Western Blot

Journal: Scientific Reports

Article Title: Passive repetitive stretching is associated with greater muscle mass and cross-sectional area in the sarcopenic muscle

doi: 10.1038/s41598-021-94709-0

Figure Lengend Snippet: Sequences of real-time PCR primers used.

Article Snippet: After blocking with a blocking buffer (Boster Biological Technology, Pleasanton, CA, USA) for 1.5 h at room temperature, the membrane was incubated with primary antibodies (diluted in TBS-T) against p-p70S6K (Thr 389) (1:1,000; A0533; Assay Biotechnology Company, Sunnyvale, CA, USA) and p70S6K (1:1,000; CSB-PA003686; CUSABIO, Houston, TX, USA), p-4E-BP1 (Thr37/46) (1:1,000; #2855; Cell Signaling technology, Danvers, MA, USA) and 4E-BP1 (1:1000; CSB-PA007994; CUSABIO), p-AKT (S473) (1:1000; CSB-PA000466; CUSABIO), AKT (1:1000; CSB-PA008132; CUSABIO), GAPDH (1:1000; CSB-PA004666; CUSABIO) for 2 h at room temperature.

Techniques: Real-time Polymerase Chain Reaction